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Erratum for Traber et al. α-Tocopherol disappearance rates from plasma depend on lipid concentrations: studies using deuterium-labeled collard greens in younger and older adults. Am J Clin Nutr 2015;101:752–9
Traber plasma deuterium-labeled collard greens
2018/11/13
Erratum for Traber et al. α-Tocopherol disappearance rates from plasma depend on lipid concentrations: studies using deuterium-labeled collard greens in younger and older adults. Am J Clin Nutr 2015;1...
2017冷泉港亚洲会议：脂质代谢和代谢紊乱（Cold Spring Harbor Asia conference on Lipid Metabolism and Metabolic Disorders）
Lipid Metabolism Metabolic Disorders
2017/1/19
We are pleased to announce the Cold Spring Harbor Asia conference on Lipid Metabolism and Metabolic Disorders which will be held in Suzhou, China, located approximately 60 miles west of Shanghai. The ...
Choose Your Label wisely:Water-Soluble Fluorophores Often Interact with Lipid Bilayers
Water Proteins Lipid Bilayers Fluorescent Dyes Spectrometry, Fluorescence Protein Binding Solubility
2016/5/25
Water-soluble organic fluorophores are widely used as labels in biological systems. However, in many cases these fluorophores can interact strongly with lipid bilayers, influencing the interaction of ...
Individual Vesicle Fusion Events Mediated by Lipid-anchored DNA
Cholesterol DNA Lipid Bilayers Phosphatidylcholines Phosphatidylethanolamines Membrane Fusion Unilamellar Liposomes
2016/5/25
Membrane fusion consists of a complex rearrangement of lipids and proteins that results in the merger of two lipid bilayers. We have developed a model system that employs synthetic DNA-lipid conjugate...
Formation and Analysis of Topographical Domains Between Lipid Membranes Tethered by DNA Hybrids of Different Lengths
Cell Membrane DNA Membrane Lipids Lipid Bilayers 1,2-Dipalmitoylphosphatidylcholine Microscopy, Fluorescence Base Sequence Molecular Sequence Data Models,Chemical Unilamellar Liposomes
2016/5/25
We recently described a strategy to prepare DNA-tethered lipid membranes either to fixed DNA on a surface or to DNA displayed on a supported bilayer [Boxer et al., J. Struct. Biol., 2009, 168, 190; Bo...
Vesicle Fusion Observed by Content Transfer Across a Tethered Lipid Bilayer
Endoplasmic reticulum-associated degradation (ERAD) ubiquitin-proteasome system (UPS) gp78/RNF45 Epidermal growth factor receptor (EGFR) Exosome Duffy antigen receptor for chemokine (DARC)
2016/5/25
Synaptic transmission is achieved by exocytosis of small, synaptic vesicles containing neurotransmitters across the plasma membrane. Here, we use a DNA-tethered freestanding bilayer as a target archit...
Stability of DNA-Tethered Lipid Membranes with Mobile Tethers
Metallic glasses Rapid-solidification Microstructure Mechanical properties
2016/5/25
We recently introduced two approaches for tethering planar lipid bilayers as membrane patches to either a supported lipid bilayer or DNA-functionalized surface using DNA hybridization (Chung, M.; Lowe...
Covalent Attachment of Lipid Vesicles to a Fluid-Supported Bilayer Allows Observation of DNA-Mediated Vesicle Interactions
Caspian and Black Sea waters reverse-osmotic desalination Na-zeolite softening of the permeate
2016/5/25
Specific membrane interactions such as lipid vesicle docking and fusion can be mediated by synthetic DNA-lipid conjugates as a model for the protein-driven processes that are ubiquitous in biological ...
Effects of Linker Sequences on Vesicle Fusion Mediated by Lipid-Anchored DNA Oligonucleotides
DNA machine synthetic biology
2016/5/25
Synthetic lipid-oligonucleotide conjugates inserted into lipid vesicles mediate fusion when one population of vesicles displays the 5'-coupled conjugate and the other the 3'-coupled conjugate, so that...
Lipid-anchored DNA Mediates Vesicle Fusion as Observed by Lipid and Content Mixing
DNA Vesicles Lipids Fuses
2016/5/25
A general method for synthesizing 5(')- and 3(')-coupled DNA-lipid conjugates has been developed and employed in DNA-mediated vesicle fusion. Vesicles presenting complementary DNA fuse, resulting in b...
Kinetics of DNA-mediated Docking Reactions Between Vesicles Tethered To Supported Lipid Bilayers
DNA electron transfer oxidative stress redox regulation
2016/5/25
Membrane-membrane recognition and binding are crucial in many biological processes. We report an approach to studying the dynamics of such reactions by using DNA-tethered vesicles as a general scaffol...
Phase Separation of Lipid Membranes Analyzed with High-Resolution Secondary Ion Mass Spectrometry
Cell Membrane Membrane Microdomains Membrane Lipids Lipid Bilayers Phosphatidylcholines Membrane Proteins Spectrometry, Mass, Secondary Ion Nanotechnology
2016/5/24
Lateral variations in membrane composition are postulated to play a central role in many cellular events, but it has been difficult to probe membrane composition and organization on length scales of t...
E-Cadherin Tethered to Micropatterned Supported Lipid Bilayers as a Model for Cell Adhesion
E-Cadherin Tethered Micropatterned Lipid Bilayers Cell Adhesion
2016/5/24
Cell-cell adhesion is a dynamic process requiring recruitment, binding, and reorganization of signaling proteins in the plane of the plasma membrane. Here, we describe a new system for investigating h...
Patterned Supported Lipid Bilayers and Monolayers on Poly(dimethylsiloxane)
cyclohexanone monooxygenase sulphide oxidation biocatalysis engineered whole cells
2016/5/24
A simple and practical method for patterning supported lipid bilayers on poly(dimethylsiloxane) is presented. By using electron microscopy grids to laterally control the extent of plasma oxidation, th...
Arrays of Mobile Tethered Vesicles on Supported Lipid Bilayers
Oligonucleotides,Antisense Lipid Bilayers Phosphatidylcholines Fluorescent Dyes Liposomes
2016/5/24
We report a new system of laterally mobile, arrayed vesicles that are encoded with DNA to control tethering to fluid-supported phospholipid bilayers. The motion of individual fluorescently labeled ves...