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微卫星PCR产物变性与非变性PAGE-银染检测方法的比较The Comparision of Denatured and non-denatured PAGE–Silver- Staining Detection of PCR Product of Microsatellites
微卫星 PAGE PCR 银染
2008/1/8
摘要
用鸡的微卫星引物对6个中国地方鸡种的两个微卫星位点进行PCR扩增。将扩增产物在变性与非变性聚丙烯酰胺凝胶（PAGE）上进行电泳，经银染，表明微卫星产物在二者上的电泳结果有明显的差异。在非变性聚丙烯酰胺凝胶中，表现为有较多的非特异带，而在变性胶中微卫星扩增产物条带清晰，易于鉴定。Abstract：The genomic DNAs from six chicken breeds in Chin...
Molecular Characterisation of Nilagiri Sheep (Ovis aries) of South India Based on Microsatellites
Genetic Characterisation Nilagiri Sheep Microsatellites PIC Inbreeding Estimates
2016/4/25
Genetic variation in Nilagiri sheep, the only apparel wool breed in South India was studied using 25 FAO recommended ovine-specific microsatellite markers. The number of observed alleles ranged from 3...