搜索结果: 1-6 共查到“知识库 生物学 RNA-seq”相关记录6条 . 查询时间(0.081 秒)
基于RNA-Seq技术苹果基因结构优化与新转录本预测
苹果 RNA-Seq 基因结构优化 新转录本预测
2018/12/13
苹果(Malus domestica)已经完成全基因组测序并公布全基因组图谱, 但是基因注释信息很不完善, 因此本试验利用RNA-Seq技术对苹果已注释基因结构进行优化和新转录本预测。以‘富士’花后15、30、45、60 d的果肉为材料提取总RNA, 构建文库并使用Illumina双末端测序HiSeq 2500平台进行测序, 获得高质量的序列(clean reads)。将得到的结果与苹果‘金冠’参...
本文以前期获得的银叶真藓在干旱-复水过程中的转录组数据为基础, 选择了9个在干旱-复水条件下基因表达差异明显的相关耐干响应基因, 并利用荧光定量PCR技术研究其在干旱-复水、ABA诱导胁迫及盐胁迫下的表达模式。研究结果表明, RT-qPCR实验获得的9个基因在干旱-复水下的表达趋势与RNA-Seq基因表达谱中基因表达规律基本一致, 因此较好地验证了RNA-Seq数据的可靠性。在干旱-复水过程中, ...
Characterizing Milk Production Related Genes in Holstein Using RNA-seq
RNA-seq Holstein Milk Production Meta-analysis Milk Yield Differentially Expressed Gene
2016/5/19
Although the chemical, physical, and nutritional properties of bovine milk have been extensively studied, only a few studies have attempted to characterize milk-synthesizing genes using RNA-seq data. ...
The simple fool’s guide to population genomics via RNA-Seq: an introduction to high-throughput sequencing data analysis
bioinformatics de novo assembly gene expression
2015/12/11
High-throughput sequencing technologies are currently revolutionizing the field of biology and medicine, yet bioinformatic challenges in analysing very large data sets have slowed the adoption o...
RNA-seq Profiles of Immune Related Genes in the Spleen of Necrotic Enteritis-afflicted Chicken Lines
RNA-seq Chicken Necrotic Enteritis Cytokine Eimeria maxima Clostridium perfringens
2016/5/19
The study aimed to compare the necrotic enteritis (NE)-induced transcriptome differences between the spleens of Marek’s disease resistant chicken line 6.3 and susceptible line 7.2 co-infected with Eim...